VRK1 interacts with p53 forming a basal complex that is activated by UV-induced DNA damage.
نویسندگان
چکیده
DNA damage immediate cellular response requires the activation of p53 by kinases. We found that p53 forms a basal stable complex with VRK1, a Ser-Thr kinase that responds to UV-induced DNA damage by specifically phosphorylating p53. This interaction takes place through the p53 DNA binding domain, and frequent DNA-contact mutants of p53, such as R273H, R248H or R280K, do not disrupt the complex. UV-induced DNA damage activates VRK1, and is accompanied by phosphorylation of p53 at Thr-18 before it accumulates. We propose that the VRK1-p53 basal complex is an early-warning system for immediate cellular responses to DNA damage.
منابع مشابه
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عنوان ژورنال:
- FEBS letters
دوره 588 5 شماره
صفحات -
تاریخ انتشار 2014